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- High numerical aperture holographic microscopy reconstruction with extended z range doi link

Auteur(s): Verrier N., Donnarumma D., Tessier G, Gross M.

(Article) Publié: Applied Optics, vol. 54 p.9540-9547 (2015)
Texte intégral en Openaccess : arxiv


Ref HAL: hal-01225788_v1
Ref Arxiv: 1511.04263
DOI: 10.1364/AO.54.009540
WoS: 000364456300025
Ref. & Cit.: NASA ADS
Exporter : BibTex | endNote
3 Citations
Résumé:

An holographic microscopy reconstruction method compatible with high numerical aperture microscope objective (MO) up to NA=1.4 is proposed. After off axis and reference field curvature corrections, and after selection of the +1 grating order holographic image, a phase mask that transforms the optical elements of the holographic setup into an afocal device is applied in the camera plane. The reconstruction is then made by the angular spectrum method. The field is first propagated in the image half space from the camera to the afocal image of the MO optimal plane (plane for which MO has been designed) by using a quadratic kernel. The field is then propagated from the MO optimal plane to the object with the exact kernel. Calibration of the reconstruction is made by imaging a calibrated object like an USAF resolution target for different positions along z. Once the calibration is done, the reconstruction can be made with an object located in any plane z. The reconstruction method has been validated experimentally with an USAF target imaged with a NA=1.4 microscope objective. Near-optimal resolution is obtained over an extended range (±50 µm) of z locations.