Sommaire:

Distinguishing edited cells from non-edited/off-target edits would transform the manufacturing of cell editing products. Visualizing the separation of bacterial colony function would open up our fundamental understanding of microbiology. Identifying chemical cues in pap smear results before the appearance of cells with odd morphologies could lead to earlier identification of dysplasia, improving patient outcomes and treatment options. In short, imaging the dynamic chemical landscape within cells and tissues would revolutionize biological discovery and medicine.
Molecular vibrational imaging techniques detect the small oscillations between bonded atoms, providing dense spectral information about composition and state without the addition of fluorophores or dyes. Technologies, such as Raman and infrared microscopies, have offered this capability for over half a century, but significant limitations in speed, resolution, or sample preparation have prevented their ubiquity.
In this presentation, I will present our developments at NIST of broadband coherent anti-Stokes Raman scattering (BCARS), a coherent Raman imaging method with an unprecedented combination of speed, sensitivity, and spectral breadth; and our development of IR microscopy for live-cell imaging, which is enabling time-course IR imaging of cell cultures at the benchtop rather than synchrotron sources.

Pour plus d'informations, merci de contacter Finco A.