Sommaire:

Examples for single cell volume and density measurements and elastography of cellular aggregates. Cell volume is a fundamental parameter for understanding cell cycle progression and regulation. I will describe a micro-fabricated system to accurately measure the volume of individual cells, adherent or not, over several cell division cycles. By following single cells through several division cycles, we found signatures of different cell size homeostasis mechanisms in different cell lines. Surprisingly, cells in mitosis exhibit a significant volume increase associated with a drop in cell density that could generate strong pushing forces.

On a multicellular level, tumors are subjected to external stress generated by their own growth in a confined environment and their surrounding tissues. Using full field optical coherence tomography, we measure spatially and temporally the strain inside spheroids after applying an isotropic osmo-mechanical pressure. The results show that spheroids do not behave as homogeneous viscoelastic spheres as they overreact to the applied stress. We finally estimate viscoelastic parameters of spheroids at the macro-scale.

Pour plus d'informations, merci de contacter Gross M.