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(1) Presentation(s)


Jeu. 15/02/2018 11:00 Grande Ourse, Bâtiment 13, Etage 1

WALLISER Nils-ole (Laboratoire Charles Coulomb)
Looping and Clustering: a statistical physics approach to protein-DNA complexes in bacteria

(Physique Théorique)


The DNA shows a high degree of spatial and dynamical organization over a broad range of length scales. It interacts with different populations of proteins and can form protein-DNA complexes that underlie various biological processes, including chromosome segregation. A prominent example is the large ParB-DNA complex, an essential component of a widely spread mechanism for DNA segregation in bacteria. Recent studies suggest that DNA-bound ParB proteins interact with each other and condense into large clusters with multiple extruding DNA-loops.

In my talk, I present the Looping and Clustering model, a simple statistical physics approach to describe how proteins assemble into a protein-DNA cluster with multiple loops. Our analytic model predicts binding profiles of ParB proteins in good agreement with data from high precision ChIP-sequencing – a biochemical technique to analyze the interaction between DNA and proteins at the level of the genome. The Looping and Clustering framework provides a quantitative tool that could be exploited to interpret further experimental results of ParB-like protein complexes and gain some new insights into the organization of DNA.

Pour plus d'informations, merci de contacter Parmeggiani A.