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Intimate effects of surface functionalization of porous silicon microcavities on biosensing performance
Auteur(s): Martin Fernandez M., Massif Laurent, Estephan Elias, Saab Marie-Belle, Cloitre T., Larroque Christian, Agarwal Vivechana, Cuisinier Frédéric, Lelay Guy, Gergely C.
Conférence invité: SPIE Optics + Photonics (San Diego, US, 2011-08-21)
Ref HAL: hal-00621199_v1
DOI: 10.1117/12.893379
WoS: 000295935600015
Exporter : BibTex | endNote
Résumé: We study the effect of different surface functionalization methods on the sensing performances of porous silicon (PSi) microcavities when used for detection of biomolecules. Previous research on porous silicon demonstrated versatility of these devices for sensor applications based on their photonic responses. The interface between biological molecules and the Si semiconductor surface is a key issue for improving biomolecular recognition in these devices. PSi microcavities were fabricated to reveal reflectivity pass-band spectra in the visible and near-infrared domain. To assure uniform infiltration of proteins the number of layers of Bragg mirrors was limited to five, the first layer being of high porosity. In one approach the devices were thermally oxidized and functionalized to assure covalent binding of molecules. Secondly, the as etched PSi surface was modified with adhesion peptides isolated via phage display technology and presenting high binding capacity for Si. Functionalization and molecular binding events were monitored via reflectometric interference spectra as shifts in the resonance peaks of the cavity structure due to changes in the refractive index when a biomolecule is attached to the large internal surface of PSi. Improved sensitivity is obtained due to the peptide interface linkers between the PSi and biological molecules compared to the silanized devices. We investigate the formation of peptide-Si interface layer via X-ray photoelectron spectroscopy, scanning tunneling microscopy and scanning electron microscopy.
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Molecular detection via hybrid peptide-semiconductor photonic devices
Auteur(s): Estephan Elias, Saab Marie-Belle, Martin Fernandez M., Cloitre T., Larroque Christian, Cuisinier Frédéric J. G., Malvezzi A. M., Gergely C.
Conference: SPIE Photonics West 2011 (, US, 2011-01-22)
Actes de conférence: SPIE Proceedings Vol. 7888, vol. p.DOI: 10.1117/12.874691 (2011)
Ref HAL: hal-00585180_v1
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Résumé: The aim of this work was to investigate the possibilities to support device functionality that includes strongly confined and localized light emission and detection processes within nano/micro-structured semiconductors for biosensing applications. The interface between biological molecules and semiconductor surfaces, yet still under-explored is a key issue for improving biomolecular recognition in devices. We report on the use of adhesion peptides, elaborated via combinatorial phage-display libraries for controlled placement of biomolecules, leading to user-tailored hybrid photonic systems for molecular detection. An M13 bacteriophage library has been used to screen 1010 different peptides against various semiconductors to finally isolate specific peptides presenting a high binding capacity for the target surfaces. When used to functionalize porous silicon microcavities (PSiM) and GaAs/AlGaAs photonic crystals, we observe the formation of extremely thin (<1nm) peptide layers, hereby preserving the nanostructuration of the crystals. This is important to assure the photonic response of these tiny structures when they are functionalized by a biotinylated peptide layer and then used to capture streptavidin. Molecular detection was monitored via both linear and nonlinear optical measurements. Our linear reflectance spectra demonstrate an enhanced detection resolution via PSiM devices, when functionalized with the Si-specific peptide. Molecular capture at even lower concentrations (femtomols) is possible via the second harmonic generation of GaAs/AlGaAs photonic crystals when functionalized with GaAs-specific peptides. Our work demonstrates the outstanding value of adhesion peptides as interface linkers between semiconductors and biological molecules. They assure an enhanced molecular detection via both linear and nonlinear answers of photonic crystals.
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Phages recognizing the Indium Nitride semiconductor surface via their peptides.
Auteur(s): Estephan Elias, Saab Marie-belle, Martin Fernandez M., Larroque Christian, Cuisinier Frédéric, Briot O., Ruffenach S., Moret M., Gergely C.
(Article) Publié:
Journal Of Peptide Science, vol. p.DOI 10.1002/psc.1315 (2010)
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AFM and Raman Studies of Graphene Exfoliated on SiC
Auteur(s): Tiberj A., Martin Fernandez M., Camara N., Poncharal Philippe, Michel T., Sauvajol J.-L., Godignon P., Camassel J.
Conference: 7th European Conference on Silicon Carbide and Related Materials (Barcelona (SPAIN), ES, 2008-09-07)
Actes de conférence: SILICON CARBIDE AND RELATED MATERIALS 2008, vol. 615-617 p.215-218 (2009)
Ref HAL: hal-00411935_v1
Exporter : BibTex | endNote
Résumé: We report an investigation of few layers graphene exfoliated on SiC. Using AFM and Raman spectroscopy, we find that the graphene thickness determined from the normalized intensity of Raman lines significantly depart from the one obtained using XPS.
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GATA3 is a master regulator of the transcriptional response to low-dose ionizing radiation in human keratinocytes.
Auteur(s): Bonin Florian, Molina Manuella, Malet Claude, Ginestet Chantal, Berthier-Vergnes Odile, Martin Fernandez M., Lamartine Jérôme
(Article) Publié:
Bmc Genomics, vol. 10 p.417 (2009)
Texte intégral en Openaccess :
Ref HAL: hal-00425191_v1
PMID 19735555
DOI: 10.1186/1471-2164-10-417
WoS: 000270394800001PubMed Central: 2753551
Exporter : BibTex | endNote
14 Citations
Résumé: BACKGROUND: The general population is constantly exposed to low levels of radiation through natural, occupational or medical irradiation. Even if the biological effects of low-level radiation have been intensely debated and investigated, the molecular mechanisms underlying the cellular response to low doses remain largely unknown. RESULTS: The present study investigated the role of GATA3 protein in the control of the cellular and molecular response of human keratinocytes exposed to a 1 cGy dose of X-rays. Chromatin immunoprecipitation showed GATA3 to be able to bind the promoter of 4 genes responding to a 1 cGy exposure. To go further into the role of GATA3 after ionizing radiation exposure, we studied the cellular and molecular consequences of radiation in GATA3 knock-down cells. Knock-down was obtained by lentiviral-mediated expression of an shRNA targeting the GATA3 transcript in differentiated keratinocytes. First, radiosensitivity was assessed: the toxicity, in terms of immediate survival (with XTT test), associated with 1 cGy radiation was found to be increased in GATA3 knock-down cells. The impact of GATA3 knock-down on the transcriptome of X-ray irradiated cells was also investigated, using oligonucleotide microarrays to assess changes between 3 h and 72 h post-irradiation in normal vs GATA3 knock-down backgrounds; transcriptome response was found to be completely altered in GATA3 knock-down cells, with a strong induction/repression peak 48 h after irradiation. Functional annotation revealed enrichment in genes known to be involved in chaperone activity, TGFbeta signalling and stress response. CONCLUSION: Taken together, these data indicate that GATA3 is an important regulator of the cellular and molecular response of epidermal cells to very low doses of radiation.
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Peptides for functionalization of InP semiconductors
Auteur(s): Estephan Elias, Saab Marie-belle, Larroque Christian, Martin Fernandez M., Olsson Fredrik, Lourdudoss Sebastian, Gergely C.
(Article) Publié:
Journal Of Colloid And Interface Science, vol. 1 p.1 (2009)
Texte intégral en Openaccess :
Ref HAL: hal-00393158_v1
PMID 19539948
DOI: 10.1016/j.jcis.2009.05.040
WoS: 000268740500007
Exporter : BibTex | endNote
24 Citations
Résumé: The challenge is to achieve high specificity in molecular sensing by proper functionalization of micro/nano-structured semiconductors by peptides that reveal specific recognition for these structures. Here we report on surface modification of the InP semiconductors by adhesion peptides produced by the phage display technique. An M13 bacteriophage library has been used to screen 1010 different peptides against the InP(001) and the InP(111) surfaces to finally isolate specific peptides for each orientation of the InP. MALDI-TOF/TOF mass spectrometry has been employed to study real affinity of the peptide towards the InP surfaces. The peptides serve for controlled placement of biotin onto InP to bind then streptavidin. Our Atomic Force Microscopy study revealed a total surface coverage of molecules when the InP surface was functionalized by its specific biotinylated peptide (YAIKGPSHFRPS). Finally, fluorescence microscopy has been employed to demonstrate the preferential attachment of the peptide onto a micro-patterned InP surface. Use of substrate specific peptides could present an alternative solution for the problems encountered in the actually existing sensing methods and molecular self-assembly due to the unwanted unspecific interactions.
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Three-dimensional spatial resolution of the nonlinear photoemission from biofunctionalized porous silicon microcavity
Auteur(s): Martin Fernandez M., Palestino Gabriela, Agarwal Vivechana, Cloitre T., Zimanyi Laszlo, Gergely C.
(Article) Publié:
Applied Physics Letters, vol. 94 p.223313 (2009)
Ref HAL: hal-00391043_v1
DOI: 10.1063/1.3148698
WoS: 000266674300080
Exporter : BibTex | endNote
6 Citations
Résumé: Infiltration of biomacromolecules into porous silicon photonic architectures results in biofunctionalized structures with unique properties. Characterization of their optical response and performance optimization in biomacromolecular detection and biophotonic application require a combination of optical and structural studies. Nonlinear optical microscopy is applied to study porous silicon microcavities with and without infiltrated glucose oxidase. The infiltrated protein acts as an internal two-photon-excited fluorescence emitter and second harmonic generator, enabling the in-depth visualization of the porous structure. Enhanced second harmonic generation and fluorescence emission by the porous silicon structure is experimentally associated with the defect layer.
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