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Biosensing and Protein Fluorescence Enhancement by Functionalized Porous Silicon Devices
Auteur(s): Palestino Gabriela, Agarwal Vivechana, Aulombard R., Perez Elias, Gergely C.
(Article) Publié:
Langmuir, vol. 24 p.13765 (2008)
Texte intégral en Openaccess :
Ref HAL: hal-00393024_v1
PMID 18959435
DOI: 10.1021/la8015707
WoS: 000261216500077
Exporter : BibTex | endNote
57 Citations
Résumé: Porous silicon (PSi) is a promising biomaterial presenting the advantage of being biocompatible and bioresorbable. Due to the large specific surface area and unique optical features, these microporous structures are excellent candidates for biosensing applications. Investigating device functionality and developing simple Si-based transducers need to be addressed in novel biological detection. Our work demonstrates that, among the various PSi configurations for molecular detection, PSi microcavity structure demonstrates the best biosensing performance, reflected through the enhanced luminescence response and the changes in the refractive index. For successful immobilization, molecular infiltration and confinement are the two key factors that are controlled by the pore size distribution of the PSi microcavities and by the surface modification obtained by silane-glutaraldehyde chemistry. Enhancement of the fluorescence emission of confined fluorescent biomolecules in the active layer of PSi microcavities was observed for a nonlabeled protein with a natural green fluorescence, the glucose oxidase enzyme (GOX). An increase in the fluorescence emission was also observed when functionalized PSi material was used to detect specific binding between biotin and a low concentration of labeled streptavidin. Evidence for the enzymatic activity ofGOXin its adsorbed form is also presented. Use of smart silicon devices, enabling enhancement of fluorescence emission of biomolecules, offers easy-to-use biosensing, based on the luminescence response of the molecules to be detected.
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Thermal and Mechanical Properties of Silicon Tetrachloride (SiCl4) and Germanium Tetrachloride (GeCl4) in Their Vapor and Liquid Phases
Auteur(s): Prat Olivier P., Cloitre T., Aulombard R.
(Article) Publié:
Chemical Vapor Deposition, vol. 13 p.199 (2007)
Ref HAL: hal-00414666_v1
Exporter : BibTex | endNote
Résumé: The two chemical species SiCl4 and GeCl4 are used, to a large extent, as starting materials in the optical fiber and semiconductor industry. The knowledge of selected physical properties of these materials (e.g. vapor pressure, viscosity, thermal conductivity, heat of vaporization, and heat capacity among others) is required for the prediction of their evaporation and deposition processes. While some of the aforementioned properties are well documented in the literature, others are seldom found or incomplete and only available for fixed temperatures. The aim of this paper is to gather the most common physical and mechanical properties for both SiCl4 and GeCl4 in their liquid and vapor phases.
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