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(135) Production(s) de CLOITRE T.
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SENSITIVE DETECTION OF H2B ANTIBODIES BY ENHANCED ELISA USING POROUS SILICON MICROCAVITIES 
Auteur(s): Ramakrishan Sathish Kumar, Martin Fernandez M., Cloitre T., Agarwal Vivechana, Cuisinier F.J.G., Gergely C.
Conference: Porous Semiconductors - Science and Technology 2018 (La Grande Motte, Montpellier, FR, 2018-03-11)
Ref HAL: hal-01745847_v1
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Résumé: We are interested in the early stage diagnosis of H2B, a clinically important autoantigen in various severe illnesses (can-cers, systemic lupus, rheumatoid arthritis). Accordingly, we have developed a porous-silicon (PSi) based enzyme-linked immunosorbent assay (ELISA) for sensitive detection of H2B antibodies, where H2B antigen were bound to a Si binding 12mer peptide [1] that act as an efficient linker to the surface. The proposed method is a simple, inexpensive sensing meth-od employing a PSi photonic substrate, a material affinity peptide, and a smart phone CCD detector to achieve a 100-fold (~ 10 fg/ml) increase in sensitivity compared to the standard ELISA (0.01 -0.1 ng/ml).
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PORE SIZE DEPENDENT CRYSTALLIZATION ON POROUS SILICON MULTILAYERS
Auteur(s): Gerecsei T., Soussi I., Cloitre T., Martin Fernandez M., Cunin Frédérique, Salehi H., Collart Dutilleul Pierre-Yves, Cuisinier Frederic J. G., Gergely C.
(Affiches/Poster)
Porous Semiconductors - Science and Technology 2018 (La Grande Motte, Montpellier, FR), 2018-03-11
Ref HAL: hal-01745839_v1
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Résumé: Mineralization of calcium phosphate crystals was examined on porous silicon (PSi) scaffolds with respect to the dependence of crystal formation on the average pore size of PSi. PSi multilayer wafers fabricated by electrochemical etching were moni- tored via their reflectivity spectra over time in a biologically relevant osteogenic solution. The evolution of the mean refractive index of the PSi scaffold is the signature of the in situ crystals nucleation within the pores. Crystals were then characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM) and Raman micro-spectroscopy. The results in- dicate that the average pore size of PSi highly influences nucleation and crystal formation.
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Elaboration and Characterization of Porous Silicon multilayer for biomaterial applications
Auteur(s): Soussi I., Mazouz Z., Martin Fernandez M., Cloitre T., Cunin Frédérique, Mghaieth R., Gergely C., Othmane A.
(Affiches/Poster)
Porous Semiconductors - Science and Technology 2018 (La Grande Motte, Montpelier, FR), 2018-03-11
Ref HAL: hal-01745799_v1
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Résumé: In this work, a combination of optical and electrochemical studies are performed in order to elaborate a suitable porous silicon (PSi) substrate for biosensing and biomaterial applications. We report on the electrochemical behavior of PSi multilayer stabilized by thermal oxidation, applying electrochemical impedance spectroscopy and cyclic voltammetry measurements. The strength of the method is evidenced when adsorption of small (12-mer) peptides is monitored. Our aim is to combine the advantages offered by the large sensing area of the PSi multilayers and the high sensitivity of the electrochemical technique for improved biosensing.
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Detection and localization of luminescent nanodiamonds in cellular environment using Raman imaging
Auteur(s): Gulka Michal, Varga B., Salehi Hamideh, Middendorp Elodie, Cloitre T., Cuisinier Frédéric J. G., Cígler Petr, Nesladek Milos, Gergely C.
Conference: MRS 2017, Fall Meeting and exhibit (, Boston, US, 2017-11-26)
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Precise nanodiamonds localization in cellular environment
Auteur(s): Gulka Michal, Varga B., Salehi Hamideh, Middendorp Elodie, Cloitre T., Cuisinier Frédéric J. G., Cígler Petr, Nesladek Milos, Gergely C.
Conference: E-MRS 2017, Fall Meeting (Warsaw, PL, 2017-09-18)
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Ellipsometric study of peptide layers – island-like character, depolarization and quasi-absorption
Auteur(s): Pápa Z., Ramakrishnan S., Martin Fernandez M., Cloitre T., Zimányi László, Tóth Z., Gergely C., Budai Judit
(Article) Publié:
Applied Surface Science, vol. p. (2017)
Ref HAL: hal-01488411_v1
DOI: 10.1016/j.apsusc.2017.02.263
WoS: 000408756700069
Exporter : BibTex | endNote
1 Citation
Résumé: In this work, the ellipsometric measurements of small molecular size polypeptides deposited onto silicon are analyzed. Results of ellipsometric evaluation procedures based on transparent layer, absorbing layer and discontinuous layer approaches are compared. Although these models result in similar fitting quality and can predict the amount of the deposited material, the gained optical properties can be rather differ- ent due to the different assumptions of the models. To choose the physically correct results, independent measurements as atomic force microscopy or transmission measurement of peptide solutions are nec- essary. It is shown that the measured ellipsometric depolarization can provide also useful information about the sample properties.
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Study of diamond particles internalization by monitoring cell membrane stiffness changes and their luminescent signal
Auteur(s): Gulka Michal, Martin Fernandez M., Salehi Hamideh, Varga B., Cloitre T., Cuisinier Frédéric J. G., Cígler Petr, Nesladek Milos, Gergely C.
(Affiches/Poster)
Hasselt Diamond Workshop 2016, SBDD XXI (Hasselt, BE), 2016-03-09
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