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(135) Production(s) de CLOITRE T.
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Detection and light enhancement of glucose oxidase adsorbed on porous silicon microcavities
Auteur(s): Palestino Gabriela, Martin Fernandez M., Agarwal Vivechana, Legros Réné, Cloitre T., Zimanyi Laszlo, Gergely C.
Conference: 6th International Conference on Porous Semiconductor Science and Technology (Mallorca, ES, 2008-03-10)
Actes de conférence: Physica Status Solidi C, vol. 6 p.1624 - 1628 (2009)
Texte intégral en Openaccess :
Ref HAL: hal-00393056_v1
DOI: 10.1002/pssc.200881006
WoS: 000279496300021
Exporter : BibTex | endNote
11 Citations
Résumé: Porous silicon (PSi) structure is used as support material to detect protein infiltration and to induce fluorescence and second harmonic light enhancement from glucose oxidase (GOX). Functionalization and protein infiltration is monitored by specular reflectometry. Optical response enhancement of PSi microcavity structures compared to PSi single layers or Bragg mirrors is observed, when GOX is impregnated. Penetration of organic molecules along the PSi microcavity structure is demonstrated by energy dispersive X-ray profile. Enhanced fluorescence emission of GOX when adsorbed on PSi microcavity is evidenced by multi-photon microscopy (MPM). Second harmonic light generation is observed at some particular pores of PSi and subsequent resonance enhancement of the signal arising from the GOX adsorbed within the pores is detected. Our work evidences an improved device functionality of GOX-PSi microcavities due to strongly confined and localized light emission within these structures. This opens the way towards the application of PSi microcavity structures as amended biosensors based on their locally enhanced optical response. The second main achievement lies in the novelty of the used techniques. In contrast to the specular reflectometry used to monitor the macroscopic optical response of PSi structures, MPM presents a valuable alternative microscopic technique probing individual pores.
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Functionalization of semiconductors for biosensing applications - art. no. 65920Z
Auteur(s): Estephan Elias, Larroque C., Martineau P., Cloitre T., Gergely C.
Conference: Conference on Bioengineered and Bioinspired Systems III (Maspalomas (SPAIN), FR, 2007-05-02)
Actes de conférence: Bioengineered and Bioinspired Systems III, vol. 6592 p.Z5920-Z5920 (2007)
Ref HAL: hal-00540381_v1
Exporter : BibTex | endNote
Résumé: Functionalization of semiconductors (SC) has been widely used for various electronic, photonic and biomedical applications. In this paper, we report on selective functionalization achieved by peptides that reveal specific recognition of the SC surfaces. A M13 bacteriophage library was used to screen 101() different 12-mer peptide on various SC substrates to successfully isolate after 3 cycles one specific peptide for the majority of semiconductors. Our results conclude that GaAs(100) and GaN(0001) retain the same sequence of 12-mer peptide, suggesting that the specificity does not depend on the crystallographic structure but it depends on the chemical composition and the electronegativity of the surface, thus on the orientation of the material. We also note the presence of at least one proline (Pro) amino acid in each peptide, and the presence of the histidine (His) in the specific peptides for the II-VI class SC. Pro imprints a constraint to the peptide to facilitate adhesion to the surface, whereas the basic side chain His is known for its affinity towards some of the elements of class II SC. Finally, fluorescence microscopy has been employed to demonstrate the preferential attachment of the peptide to their specific SC surface in close proximity to a surface of different chemical and structural composition. The use of selected peptides expressed by phage display can be extended to encompass a variety of nanostructured semiconductor based devices.
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Thermal and Mechanical Properties of Silicon Tetrachloride (SiCl4) and Germanium Tetrachloride (GeCl4) in Their Vapor and Liquid Phases
Auteur(s): Prat Olivier P., Cloitre T., Aulombard R.
(Article) Publié:
Chemical Vapor Deposition, vol. 13 p.199 (2007)
Ref HAL: hal-00414666_v1
Exporter : BibTex | endNote
Résumé: The two chemical species SiCl4 and GeCl4 are used, to a large extent, as starting materials in the optical fiber and semiconductor industry. The knowledge of selected physical properties of these materials (e.g. vapor pressure, viscosity, thermal conductivity, heat of vaporization, and heat capacity among others) is required for the prediction of their evaporation and deposition processes. While some of the aforementioned properties are well documented in the literature, others are seldom found or incomplete and only available for fixed temperatures. The aim of this paper is to gather the most common physical and mechanical properties for both SiCl4 and GeCl4 in their liquid and vapor phases.
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Functionalization of semiconductors for biosensing applications
Auteur(s): Estephan Elias, Larroque Christian, Martineau P., Cloitre T., Gergely C.
Conference: SPIE Europe Microtechnologies for the new millenium (Maspalomas, Gran Canaria, ES, 2007-05-02)
Actes de conférence: Proceedings SPIE, vol. 6592 p.65920Z1-9 (2007)
Ref HAL: hal-00393670_v1
DOI: 10.1117/12.721906
WoS: 000250170700032
Exporter : BibTex | endNote
1 Citation
Résumé: Functionalization of semiconductors (SC) has been widely used for various electronic, photonic and biomedical applications. In this paper, we report on selective functionalization achieved by peptides that reveal specific recognition of the SC surfaces. A M13 bacteriophage library was used to screen 1010 different 12-mer peptide on various SC substrates to successfully isolate after 3 cycles one specific peptide for the majority of semiconductors. Our results conclude that GaAs(100) and GaN(0001) retain the same sequence of 12-mer peptide, suggesting that the specificity does not depend on the crystallographic structure but it depends on the chemical composition and the electronegativity of the surface, thus on the orientation of the material. We also note the presence of at least one proline (Pro) amino acid in each peptide, and the presence of the histidine (His) in the specific peptides for the II-VI class SC. Pro imprints a constraint to the peptide to facilitate adhesion to the surface, whereas the basic side chain His is known for its affinity towards some of the elements of class II SC. Finally, fluorescence microscopy has been employed to demonstrate the preferential attachment of the peptide to their specific SC surface in close proximity to a surface of different chemical and structural composition. The use of selected peptides expressed by phage display can be extended to encompass a variety of nanostructured semiconductor based devices.
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