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- Physico-chemical properties and surface characterization of renewable hybrid nanofilms interacting with model proteins doi link

Auteur(s): Theodoratou A., Costa Luca, Bonnet L., Blanc C., Lapinte Vincent, Etienne P., Milhiet Pierre-Emmanuel, Robin Jean-Jacques, Oberdisse J., Chopineau Joel, Aubert-Pouessel Anne

(Article) Publié: European Polymer Journal, vol. 111 p.161-169 (2019)
Texte intégral en Openaccess : openaccess


Ref HAL: hal-01974331_v1
DOI: 10.1016/j.eurpolymj.2018.12.018
WoS: 000458597600018
Exporter : BibTex | endNote
2 Citations
Résumé:

A comprehensive characterization of physical-chemical properties and biological interactions of ca. 200-nm-thick hybrid films based on silylated (inorganic part) castor oil (organic part) is proposed. A series of such nanofilms was fabricated and cross-linked by a sol-gel procedure, and their properties such as hydrophilicity, hardness and water vapour transmission rate were systematically studied as a function of the ratio of silylated agent to castor oil. It was found that the nanofilms have contact angles always below 90°, tunable Young modulus and hardness in the MPa range. Moreover, their water vapour transmission rates are increased by decreasing the silica ratio. The protein adsorption and cytocompatibility were evaluated using model proteins and cells. The adsorption of the proteins bovine serum albumin (BSA) and lysozyme was characterized using a quartz crystal microbalance in energy dissipation mode (QCM-D), and atomic force microscopy (AFM). The combination of the latter provided evidence for the different affinities of the proteins with the films. It was found that BSA and lysozyme form rigid layers on the surface with surface coverage close to 30%, and that both protein layers decrease their thickness after their dehydration. Finally, cell culture experiments exhibited a good viability of the fibroblasts compared to ultra-low adhesion surfaces, which makes them potential candidates for biomedical applications.